cytotoxicity effect of cladribine on the mcf-7 human breast cancer cell line
نویسندگان
چکیده
cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-cda) on the breast cancer cell line, mcf-7 (estrogen receptor positive, er+). mtt assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apoptosis. the activation of caspase-3 and -9 was assayed using caspase activation assay kits. gel electrophoresis was performed to detect dna fragmentation. treatment of mcf-7 cells with different concentrations of 2-cda resulted in a significant increase in the cell death. annexin v-fluorescein/pi and hoechst 33258 staining revealed that the cell death was mainly an apoptotic type. a significant (p<0.05) increase in the activity of caspase-9 was observed but caspase-3 activity was unchanged and dna laddering profile was not obtained. pre-treatment of the cells with kinase inhibitor, 5¢-amino-5¢-deoxyadenosine inhibited the cytotoxicity effect of cladribine. in conclusion, this study has shown that high dose of cladribine (higher than 25 μm) has an apoptotic effect on mcf-7 cells and that its intracellular phosphorylation is necessary
منابع مشابه
Cytotoxicity Effect of Cladribine on the MCF-7 Human Breast Cancer Cell Line
Cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-CdA) on the breast cancer cell line, MCF-7 (estrogen receptor positive, ER+). MTT assay, annexin V-Fluorescein/PI and Hoechst 33...
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Cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-CdA) on the breast cancer cell line, MCF7 (estrogen receptor positive, ER). MTT assay, annexin V-Fluorescein/PI and Hoechst 3325...
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عنوان ژورنال:
iranian biomedical journalجلد ۸، شماره ۱، صفحات ۷-۱۲
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